AmScope B120C
AmScope B120C Series Binocular Compound Microscope User Manual
Model: B120C | Brand: AmScope
1. Prodotto finitoview
The AmScope B120C compound binocular microscope is designed for both student and professional use, offering a wide magnification range from 40X to 2500X. It features a comfortable Siedentopf head, precise 3D mechanical stage, and bright LED illumination, making it suitable for detailed examination of various specimens.

Figure 1.1: The AmScope B120C Binocular Compound Microscope, showcasing its main components including the binocular head, objective lenses, mechanical stage, and included eyepieces and immersion oil.
- Magnification Excellence: Offers a broad magnification range from 40X to 2500X for detailed specimen examination.
- Advanced LED Illumination: Provides bright, daylight-balanced illumination with a specialized fly-eye lens for optimal viewing.
- Design ergonomico: Features a 360-degree rotatable Siedentopf head with adjustable interpupillary distance for comfortable and flexible viewing.
- Precisione Stage Controllo: A 3D mechanical stage and coaxial coarse and fine focus knobs allow for smooth and precise adjustments.
2. Cosa c'è nella scatola
Dopo aver disimballato il prodotto, verificare che siano presenti tutti i seguenti componenti:
- AmScope B120C microscope with double-layer mechanical stage
- (4) DIN Standard Objectives: 4x, 10x, 40xS, and 100xS (oil)
- 10x widefield eyepieces, one pair
- 25x widefield eyepieces, one pair
- Color filter, blue
- Immersion oil, one bottle
- Copertura antipolvere
- Istruzioni (questo manuale)
3. Guida all'installazione
Follow these steps to set up your AmScope B120C microscope:
- Disimballaggio: Rimuovere con cautela tutti i componenti dall'imballaggio. Conservare l'imballaggio originale per un eventuale stoccaggio o trasporto futuro.
- Posizionamento: Place the microscope on a stable, level surface away from direct sunlight, excessive heat, or vibrations.
- Installare gli oculari: Insert the desired pair of eyepieces (10x or 25x) into the ocular tubes of the Siedentopf head.
- Connetti alimentazione: Plug the power cord into the microscope's power input and then into a standard electrical outlet (100 to 240VAC 50/60Hz).
- Copertura antipolvere: Keep the dust cover on the microscope when not in use to protect it from dust and debris.
Video 3.1: Un overview of the AmScope B120C Siedentopf Binocular Compound Microscope, demonstrating its features and basic setup. This video is provided by United Scope LLC, the manufacturer.
4. Istruzioni per l'uso
4.1. Regolazione dell'ingrandimento
The microscope offers a wide range of magnification by combining different eyepieces and objective lenses. The total magnification is calculated by multiplying the eyepiece magnification by the objective lens magnification.
- Oculari: Use either the 10x widefield or 25x widefield eyepieces.
- Lenti dell'obiettivo: Rotate the forward-facing nosepiece to select from 4x, 10x, 40xS (spring-loaded), and 100xS (spring-loaded, oil immersion) DIN achromatic objectives.
- The 40xS and 100xS objectives are spring-loaded to prevent damage to slides when focusing.

Figure 4.1: Detail of the objective lenses (4x, 10x, 40x, 100x) and the additional WF25X eyepieces, illustrating the microscope's magnification capabilities.
4.2. Controllo dell'illuminazione
The microscope uses lower (diascopic) Brightfield LED illumination to transmit light through the specimen.
- Sorgente luminosa a LED: Turn on the LED illumination using the power switch. Adjust the brightness using the rheostat control knob.
- Abbe Condenser (1.25 NA): Adjust the condenser to control the distance of the light from the stage.
- Diaframma a iride: Use the iris diaphragm lever to optimize the amount of light illuminating the specimen, enhancing contrast and resolution.

Figure 4.2: The LED transmitted illumination system, providing bright and cool light for viewing specimens from below the stage.
4.3. Messa a fuoco
Achieve clear focus using the coaxial coarse and fine focus knobs:
- Manopola di messa a fuoco grossolana: Use for large adjustments to bring the specimen into approximate focus, especially with lower magnification objectives.
- Manopola di messa a fuoco fine: Use for small, precise adjustments to achieve sharp focus, particularly with higher magnification objectives.

Figura 4.3: La meccanica stage and coaxial coarse and fine focus knobs, designed for smooth and accurate specimen positioning and focusing.
4.4. Meccanico Stage Operazione
The double-layer mechanical stage allows for precise movement of the slide.
- Slide Holder: Secure the microscope slide using the slide clips on the mechanical stage.
- X-Y Axis Control: Use the X-Y axis knobs located below the stage to move the slide horizontally and vertically, allowing you to scan the specimen area.
4.5. Siedentopf Head Adjustment
The Siedentopf binocular head is designed for comfortable viewing e condivisione.
- Inter-pupillary Adjustment: Adjust the distance between the two ocular tubes (53 to 77mm) to match your eye spacing for comfortable viewing.
- Regolazione diottrica: Use the diopter ring on one of the ocular tubes to compensate for differences in eye strength between your left and right eyes.
- Rotazione: The head can rotate 360 degrees, which is useful for sharing the view with others without moving the entire microscope.

Figure 4.4: The 360-degree rotatable Siedentopf binocular head, angled at 30 degrees for ergonomic viewing and easy sharing.
5. Manutenzione
Una corretta manutenzione garantisce la longevità e le prestazioni ottimali del microscopio:
- Pulizia delle lenti: Per pulire obiettivi e oculari, utilizzare solo carta e soluzione specifiche per la pulizia delle lenti. Evitare di toccare le superfici ottiche a mani nude.
- Pulizia del corpo: Pulisci il corpo del microscopio con un panno morbido eamp stoffa. Evitare l'uso di prodotti chimici aggressivi o detergenti abrasivi.
- Protezione dalla polvere: Always use the provided dust cover when the microscope is not in use to prevent dust accumulation.
- Magazzinaggio: Conservare il microscopio in un luogo fresco e asciutto, lontano dalla luce solare diretta e da temperature estreme.
- Olio da immersione: Dopo aver utilizzato l'obiettivo a immersione in olio 100x, pulire immediatamente l'olio dalla lente utilizzando carta per lenti e soluzione detergente.
6. Risoluzione Dei Problemi
Questa sezione affronta i problemi più comuni che potresti incontrare:
| Problema | Possibile causa | Soluzione |
|---|---|---|
| Nessuna illuminazione | Power cord not connected, power switch off, bulb failure, fuse blown. | Check power connection. Turn on power switch. Replace LED bulb (contact support if needed). Check/replace fuse. |
| L'immagine è sfocata o fuori fuoco | Incorrect focus adjustment, dirty lenses, objective not fully clicked into place. | Adjust coarse and fine focus. Clean objective and eyepiece lenses. Rotate objective until it clicks into position. |
| Dark spots in the field of view | Dust on eyepiece, objective, or condenser. | Clean eyepieces and objectives. Check for dust on the condenser and stage. |
| Double image when using binocular head | Inter-pupillary distance not correctly adjusted. | Adjust the inter-pupillary distance until a single circular field of view viene osservato. |
7. Specifiche
| Caratteristica | Dettaglio |
|---|---|
| Testa | Compound binocular, Siedentopf, 30-degree inclination, 360-degree rotatable |
| Oculari | WF 10x, WF 25x |
| Obiettivi | 4X, 10x, 40xS, 100xS (oil) DIN achromatic |
| Gamma di ingrandimento | 40X-2500X |
| Stage | Doppio strato meccanico Stage con divisioni da 1 mm |
| Messa a fuoco | Manopole coassiali di messa a fuoco grossolana e fine |
| Configurazione dell'illuminazione | Transmitted (bottom) Brightfield |
| Condensatore | 1.25 NA Abbe with iris diaphragm |
| Sorgente luminosa | GUIDATO |
| Energia | 100 to 240VAC 50/60Hz |
| Dimensioni del prodotto | 18 x 12 x 10 pollici |
| Peso dell'articolo | 3.25 libbre |
| Materiale | Enamel-coated cast-steel body |
8. Garanzia e supporto
AmScope products are manufactured by United Scope LLC, a company founded in 1996 and headquartered in Irvine, CA. For technical support, warranty information, or service inquiries, please contact AmScope customer service directly. Refer to the official AmScope websito per i dettagli di contatto e le politiche di garanzia più aggiornati.
Risorse online:
- Official AmScope Websito: www.amscope.com
- Product Support Page: Check the AmScope website for specific support pages related to the B120C model.
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